Fig. 3

G-actin content and F-/G-actin ratio of the alveolar macrophage subpopulations. a G-actin and cell membrane stain of the alveolar macrophage subpopulations. Cells were fixed, permeabilized, and stained with Alexa Fluor 488-conjugated phalloidin, Alexa Fluor 594-conjugated DNase I, and Alexa Fluor 647-wheat germ agglutinin. Images were acquired sequentially with a Leica SP8 AOBS laser scanning confocal system with software-adjusted detection spectra to avoid bleed-through of the signals. The pseudocolors were assigned by ImageJ. Scale bars 10μΜ. b Means of G-actin signal intensity per phenotype. Total number of cells, n = 286. c Means of F-/G-actin ratio per phenotype. All cells from b were used for the measurements. Comparisons were made by two-tailed t test with Welch’s correction (equal variances not assumed). *P ≤ 0.05; **P ≤ 0.01; ****P ≤ 0.0001