Table 6 Two-way ANOVA for univariate and pairwise comparisons between gender, estrous cycle stages, and exposure for STAT3 Tyrosine 705 phosphorylation expression
From: Sex-specific IL-6-associated signaling activation in ozone-induced lung inflammation
Univariate tests: dependent variable: STAT3 Tyrosine 705 | |||||||
|  |  |  | df | F | Sig. | Partial η2 | |
Analysis of gender and exposure | Exposure | FA | 1, 52 | 0.001 | 1.000 | 0.001 | |
Ozone | 1, 52 | 24.800 | <0.0005 | 0.323 | |||
Sex | F | 1, 52 | 60.448 | <0.0005 | 0.538 | ||
M | 1, 52 | 162.686 | <0.0005 | 0.758 | |||
Analysis of female estrous cycle stages and exposure | Estrous cycle stage | Non-proestrus | 1, 28 | 58.045 | <0.0005 | 0.675 | |
Proestrus | 1, 28 | 50.192 | <0.0005 | 0.642 | |||
Exposure | FA | 1, 28 | 15.759 | <0.0005 | 0.360 | ||
Ozone | 1, 28 | 11.804 | 0.002 | 0.297 | |||
Pairwise comparisons: dependent variable: STAT3 Tyrosine 705 | |||||||
|  |  |  | (I) Sex | (J) Sex | Mean difference (I–J) | 95 % CI for difference | |
Lower bound | Upper bound | ||||||
Analysis of gender and exposure | Exposure | FA | M | F | 1.429E−8 | 0.663 | 0.663 |
Ozone | M | F | 1.645 | 0.982 | 2.308 | ||
Sex | F | Ozone | FA | 2.569 | 1.906 | 3.232 | |
M | Ozone | FA | 4.214 | 3.551 | 4.877 | ||
Analysis of female estrous cycle stages and exposure | Estrous cycle stage | Non-proestrus | Ozone | FA | 1.185 | 0.866 | 1.504 |
Proestrus | Ozone | FA | 1.102 | 0.783 | 1.421 | ||
Exposure | FA | Proestrus | Non-proestrus | 0.617 | 0.299 | 0.936 | |
Ozone | Proestrus | Non-proestrus | 0.534 | 0.216 | 0.853 | ||