Table 5 Two-way ANOVA for univariate and pairwise comparisons between gender, estrous cycle stages, and exposure for STAT3 Serine 727 phosphorylation expression
From: Sex-specific IL-6-associated signaling activation in ozone-induced lung inflammation
Univariate tests: dependent variable: STAT3 Serine 727 | |||||||
|  |  |  | df | F | Sig. | Partial η2 | |
Analysis of gender and exposure | Exposure | FA | 1, 60 | 0.001 | 1.000 | 0.002 | |
Ozone | 1,60 | 27.137 | <0.0005 | 0.311 | |||
Sex | F | 1, 60 | 17.209 | <0.0005 | 0.223 | ||
M | 1, 60 | 1.126 | 0.293 | 0.018 | |||
Analysis of female estrous cycle stages and exposure | Estrous cycle stage | Non-proestrus | 1, 28 | 2.282 | 0.142 | 0.075 | |
Proestrus | 1, 28 | 53.117 | <0.0005 | 0.655 | |||
Exposure | FA | 1, 28 | 9.535 | 0.005 | 0.254 | ||
Ozone | 1, 28 | 141.295 | <.0005 | 0.835 | |||
Pairwise comparisons: dependent variable: STAT3 Serine 727 | |||||||
|  |  |  | (I) Sex | (J) Sex | Mean difference (I–J) | 95 % CI for difference | |
Lower bound | Upper bound | ||||||
Analysis of gender and exposure | Exposure | FA | F | M | 2.500E-8 | 0.388 | 0.388 |
Ozone | F | M | 1.009 | 0.622 | 1.397 | ||
Sex | F | Ozone | FA | 0.804 | 0.416 | 1.191 | |
M | FA | Ozone | 0.206 | 0.182 | 0.593 | ||
Analysis of female estrous cycle stages and exposure | Estrous cycle stage | Non-proestrus | FA | Ozone | 0.254 | 0.090 | 0.597 |
Proestrus | Ozone | FA | 1.223 | 0.880 | 1.567 | ||
Exposure | FA | Proestrus | Non-proestrus | 0.518 | 0.174 | 0.862 | |
Ozone | Proestrus | Non-proestrus | 1.995 | 1.652 | 2.339 | ||