Fig. 5

a Relaxation induced by bradykinin following pre-constriction with U46619 following incubation with MgSO₄ (0.2 mmol/l), INDO (10 μmol/l), TRAM-34 (1 μM) and apamin (3 μM) in vessels isolated from male term pregnancies (empty circle) n = 10, female term pregnancies (empty triangle) n = 10, male preterm pregnancies (filled circle) n = 7 and female preterm pregnancies (filled triangle) n = 7. Data was analysed using a repeated measures three-way ANOVA using treatment × term × sex as the main factors. All observations were made in a minimum of two vessels per placenta, and the average responses were calculated before further statistical analysis. n refers to the number of placenta used. All data are presented as means ± SEM. b Relaxation induced by bradykinin following pre-constriction with U46619 following incubation with MgSO4 (0.2 mmol/l) and indomethacin (10 μmol/l) in vessels isolated from male term pregnancies (empty circle) n = 10, female term pregnancies (empty triangle) n = 10, male preterm pregnancies (filled circle) n = 7 and female preterm pregnancies (filled triangle) n = 7. Data was analysed using a repeated measures three-way ANOVA using treatment x term x sex as main factors. All observations were made in a minimum of two vessels per placenta and the average responses calculated before further statistical analysis. n refers to the number of placenta used. All data are means ± SEM. c Relaxation induced by bradykinin following pre-constriction with U46619 following incubation with MgSO₄ (0.2 mmol/l), TRAM-34 (1 μM) and Apamin (3 μM) in vessels isolated from male term pregnancies (empty circle) n = 10, female term pregnancies (empty triangle) n = 10, male preterm pregnancies (filled circle) n = 7 and female preterm pregnancies (filled triangle) n = 7. Data was analysed using a repeated measures three-way ANOVA using treatment × term × sex as the main factors. All observations were made in a minimum of two vessels per placenta and the average responses calculated before further statistical analysis. n refers to the number of placenta used. All data are presented as means ± SEM