Figure 1

Typical fluorescence dot plot and correlation of two distinct antibodies for platelet-specific antigens. Left, typical fluorescence dot plot (Q; quadrant) from the MV gate of the light scatter by FACSCanto™ flow cytometer showing the spectra for CD41and CD42a positive MV of activated platelets from apparently healthy women and men. All (100%) platelet-derived MV did not carry both CD41and CD42a antigens (Q2). Right, correlation of two distinct antibodies for platelet-specific antigens [CD41 (Q4+Q2) and CD42a (Q1+Q2)] binding to MV isolated from the same blood sample of apparently healthy women and men. A similar pattern was observed using two distinct antibodies for other cell-specific identification of the MV (data not shown).