Figure 5

DES treatment increases BAT Bmp8b expression in female mice. (A) Uterus weight as percent of body weight (BW) of female mice 2 weeks following gonadectomy (GDX) or sham operation and exposure to daily injections with diethylstilbestrol (DES) or olive oil vehicle during the last week. (B) Ucp1, Bmp8b, and Fgf1 mRNA expression in BAT of female mice 2 weeks following gonadectomy (GDX) or sham operation and exposure to daily injections with diethylstilbestrol (DES) or olive oil vehicle during the last week. Results were normalized to 18S ribosomal RNA (Rn18s) and beta-2 microglobulin (B2m) with data from placebo or vehicle mice defined as ‘1’. Values are averages ± SEM; n = 6; †p < 0.025 (Mann–Whitney U post hoc test when significance was detected by two-way ANOVA). Depicted below the graphs are the significant p values of the two-way ANOVA tests for either treatment (T), GDX (G), or the interaction between treatment and GDX (TxG).