Table 1 Statistics for experiment 1

Clinical scores

Figure 1A

Day: F_23,506 = 25.1, P < 0.001

Figure 1B

Day: F_23,437 = 14.3, P < 0.001

Day: F_23,943 = 37.1, P < 0.001

Treatment: F_1,22 = 9.0, P < 0.01

Treatment: F_1,19 = 8.6, P < 0.01

Treatment: F_1,41 = 16.9, P < 0.001

Day × treatment: F_23,506 = 3.2, P < 0.05

Day × treatment: F_23,437 = 2.2, P < 0.01

Sex: F_1,41 = 0.5, P = 0.4

Treatment × sex: F_1,41 = 0.2, P = 0.6

Day × treatment: F_23,943 = 2.8, P < 0.01

Day × sex: F_23,943 = 1.3, P = 0.2

Day × sex × treatment: F_23,943 = 0.4, P = 0.8

Grip strength

Figure 1C

Day: F_9,198 = 3.3, P < 0.001

Figure 1D

Day: F_9,171 = 3.7, P < 0.001

Day: F_9,369 = 5.8, P < 0.001

Treatment: F_1,22 = 2.0, P = 0.17

Treatment: F_1,19 = 7.3, P < 0.05

Treatment: F_1,41 = 8.2, P < 0.01

Day × treatment: F_9,198 = 1.2, P = 0.3

Day × treatment: F_9,171 = 1.3, P = 0.2

Sex: F_1,41 = 32.7, P < 0.001

Treatment × sex: F_1,41 = 0.7, P = 0.4

Day × treatment: F_9,369 = 1.9, P < 0.05

Day × sex: F_9,369 = 1.2, P = 0.2

Day × sex × treatment: F_9,369 = 0.6, P = 0.7

Weight

Figure 1E

Day: F_22,484 = 16.0, P < 0.001

Figure 1 F

Day: F_22,418 = 49.7, P < 0.001

Day: F_22,902 = 54.4, P < 0.001

Treatment: F_1,22 = 0.2, P = 0.6

Treatment: F_1,19 = 0.06, P = 0.8

Treatment: F_1,41 = 0.2, P = 0.6

Day × treatment: F_22,484 = 0.8, P = 0.5

Day × treatment: F_22,418 = 0.6, P = 0.9

Sex: F_1,41 = 1.4, P = 0.2

Treatment × sex: F_1,41 = 0.005, P = 0.9

Day × treatment: F_22,902 = 0.6, P = 0.7

Day × sex: F_22,902 = 3.2, P < 0.01

Day × sex × treatment: F_22,902 = 0.7, P = 0.6

Mechanical thresholds

Figure 2A

Day: F_9,198 = 4.2, P < 0.001

Figure 2B

Day: F_9,171 = 2.0, P < 0.05

Day: F_9,369 = 4.8, P < 0.001

Treatment: F_1,22 = 13.8, P < 0.01

Treatment: F_1,19 = 1.3, P = 0.2

Treatment: F_1,41 = 10.2, P < 0.01

Day × treatment: F_9,198 = 4.5, P < 0.001

Day × treatment: F_9,171 = 0.5, P = 0.8

Sex: F_1,41 = 7.3, P < 0.05

Treatment × sex: F_1,41 = 1.6, P = 0.2

Day × treatment: F_9,369 = 2.8, P < 0.01

Day × sex: F_9,369 = 0.8, P = 0.5

Day × sex × treatment: F_9,369 = 1.1, P = 0.3

Acetone withdrawal

Figure 2C

Day: F_9,198 = 14.5, P < 0.001

Figure 2D

Day: F_9,171 = 7.0, P < 0.001

Day: F_9,369 = 20.2, P < 0.001

Treatment: F_1,22 = 6.5, P < 0.05

Treatment: F_1,19 = 0.02, P = 0.8

Treatment: F_1,41 = 2.7, P = 0.16

Day × treatment: F_9,198 = 8.2, P < 0.001

Day × treatment: F_9,171 = 0.6, P = 0.6

Sex: F_1,41 = 22.5, P < 0.001

Treatment × sex: F_1,41 = 3.4, P = 0.07

Day × treatment: F_9,369 = 4.3, P < 0.001

Day × sex: F_9,369 = 1.6, P = 0.13

Day × sex × treatment: F_9,369 = 5.2, P < 0.001

Heat latency

Figure 2E

Day: F_3,66 = 0.06, P = 0.9

Figure 2F

Day: F_3,57 = 1.8, P = 0.14

Day: F_3,123 = 1.052, P = 0.3

Treatment: F_1,22 = 0.7, P = 0.3

Treatment: F_1,19 = 0.2, P = 0.5

Treatment: F_1,41 = 0.01, P = 0.8

Day × treatment: F_3,66 = 1.8, P = 0.15

Day × treatment: F_3,57 = 0.2, P = 0.8

Sex: F_1,41 = 2.1, P = 0.14

Treatment × sex: F_1,41 = 0.9, P = 0.3

Day × treatment: F_3,123 = 1.5, P = 0.19

Day × sex: F_3,123 = 1.1, P = 0.3

Day × sex × treatment: F_3,123 = 0.3, P = 0.8

AUC

Figure 2G

Mechanical thresholds: one-way ANOVA: F_3,41 = 11.1, P < 0.001

Figure 2H

Acetone withdrawal: one-way ANOVA: F_3,41 = 13.2, P < 0.001

Heat latency: one-way ANOVA: F_3,41 = 0.8, P = 0.4 (Figure 2I)