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Table 1 Statistics for experiment 1

From: Sex differences in a mouse model of multiple sclerosis: neuropathic pain behavior in females but not males and protection from neurological deficits during proestrus

Measure Two-way RM ANOVA (treatment × time) Three-way RM ANOVA (treatment × sex × time)
Females Males
Figure   Figure  
Clinical scores Figure 1A Day: F23,506 = 25.1, P < 0.001 Figure 1B Day: F23,437 = 14.3, P < 0.001 Day: F23,943 = 37.1, P < 0.001
Treatment: F1,22 = 9.0, P < 0.01 Treatment: F1,19 = 8.6, P < 0.01 Treatment: F1,41 = 16.9, P < 0.001
Day × treatment: F23,506 = 3.2, P < 0.05 Day × treatment: F23,437 = 2.2, P < 0.01 Sex: F1,41 = 0.5, P = 0.4
   Treatment × sex: F1,41 = 0.2, P = 0.6
Day × treatment: F23,943 = 2.8, P < 0.01
Day × sex: F23,943 = 1.3, P = 0.2
Day × sex × treatment: F23,943 = 0.4, P = 0.8
Grip strength Figure 1C Day: F9,198 = 3.3, P < 0.001 Figure 1D Day: F9,171 = 3.7, P < 0.001 Day: F9,369 = 5.8, P < 0.001
Treatment: F1,22 = 2.0, P = 0.17 Treatment: F1,19 = 7.3, P < 0.05 Treatment: F1,41 = 8.2, P < 0.01
Day × treatment: F9,198 = 1.2, P = 0.3 Day × treatment: F9,171 = 1.3, P = 0.2 Sex: F1,41 = 32.7, P < 0.001
   Treatment × sex: F1,41 = 0.7, P = 0.4
Day × treatment: F9,369 = 1.9, P < 0.05
Day × sex: F9,369 = 1.2, P = 0.2
Day × sex × treatment: F9,369 = 0.6, P = 0.7
Weight Figure 1E Day: F22,484 = 16.0, P < 0.001 Figure 1 F Day: F22,418 = 49.7, P < 0.001 Day: F22,902 = 54.4, P < 0.001
Treatment: F1,22 = 0.2, P = 0.6 Treatment: F1,19 = 0.06, P = 0.8 Treatment: F1,41 = 0.2, P = 0.6
Day × treatment: F22,484 = 0.8, P = 0.5 Day × treatment: F22,418 = 0.6, P = 0.9 Sex: F1,41 = 1.4, P = 0.2
   Treatment × sex: F1,41 = 0.005, P = 0.9
Day × treatment: F22,902 = 0.6, P = 0.7
Day × sex: F22,902 = 3.2, P < 0.01
Day × sex × treatment: F22,902 = 0.7, P = 0.6
Mechanical thresholds Figure 2A Day: F9,198 = 4.2, P < 0.001 Figure 2B Day: F9,171 = 2.0, P < 0.05 Day: F9,369 = 4.8, P < 0.001
Treatment: F1,22 = 13.8, P < 0.01 Treatment: F1,19 = 1.3, P = 0.2 Treatment: F1,41 = 10.2, P < 0.01
Day × treatment: F9,198 = 4.5, P < 0.001 Day × treatment: F9,171 = 0.5, P = 0.8 Sex: F1,41 = 7.3, P < 0.05
   Treatment × sex: F1,41 = 1.6, P = 0.2
      Day × treatment: F9,369 = 2.8, P < 0.01
      Day × sex: F9,369 = 0.8, P = 0.5
      Day × sex × treatment: F9,369 = 1.1, P = 0.3
Acetone withdrawal Figure 2C Day: F9,198 = 14.5, P < 0.001 Figure 2D Day: F9,171 = 7.0, P < 0.001 Day: F9,369 = 20.2, P < 0.001
Treatment: F1,22 = 6.5, P < 0.05 Treatment: F1,19 = 0.02, P = 0.8 Treatment: F1,41 = 2.7, P = 0.16
Day × treatment: F9,198 = 8.2, P < 0.001 Day × treatment: F9,171 = 0.6, P = 0.6 Sex: F1,41 = 22.5, P < 0.001
   Treatment × sex: F1,41 = 3.4, P = 0.07
Day × treatment: F9,369 = 4.3, P < 0.001
Day × sex: F9,369 = 1.6, P = 0.13
Day × sex × treatment: F9,369 = 5.2, P < 0.001
Heat latency Figure 2E Day: F3,66 = 0.06, P = 0.9 Figure 2F Day: F3,57 = 1.8, P = 0.14 Day: F3,123 = 1.052, P = 0.3
Treatment: F1,22 = 0.7, P = 0.3 Treatment: F1,19 = 0.2, P = 0.5 Treatment: F1,41 = 0.01, P = 0.8
Day × treatment: F3,66 = 1.8, P = 0.15 Day × treatment: F3,57 = 0.2, P = 0.8 Sex: F1,41 = 2.1, P = 0.14
   Treatment × sex: F1,41 = 0.9, P = 0.3
Day × treatment: F3,123 = 1.5, P = 0.19
Day × sex: F3,123 = 1.1, P = 0.3
Day × sex × treatment: F3,123 = 0.3, P = 0.8
AUC Figure 2G Mechanical thresholds: one-way ANOVA: F3,41 = 11.1, P < 0.001 Figure 2H Acetone withdrawal: one-way ANOVA: F3,41 = 13.2, P < 0.001 Heat latency: one-way ANOVA: F3,41 = 0.8, P = 0.4 (Figure 2I)
  1. Treatment conditions: CFA (females n = 13, males n = 10), CFA + MOG35-55 (females n = 11, males n = 11). AUC area under the curve.