Figure 2

Sex specific expression of the X/Y paralogues Kdm5c and Kdm5d . Three PCR primer pairs have been designed for recognizing specifically either Kdm5c or Kdm5d cDNA and for recognizing both Kdm5c/5d cDNA. Their expression was studied in male and female placentas in pregnant female mice fed either a control diet (CD) or a high-fat diet (HFD) from E0.5 to sacrifice at E15.5 stage. Kdm5c expression is higher in females (pink bars) than males (blue bars), and Kdm5d is expressed only in males, regardless of maternal diet. The Kdm5c/5d PCR shows that the combined expression of Kdm5d and Kdm5c expression in males is not of equivalent magnitude as the expression of Kdm5c from both alleles in females.