Hypothalamic kisspeptin mRNA expression in peripubertal females. (A) Representative autoradiographs depicting Kiss1 mRNA signal in the AVPV of periputertal females (Experiment 2). (B) Expression was significantly higher in the control (OIL) group compared to the PPT, DPN+PPT and EB groups at both PND24 and 33. (C) Within the ARC, expression was only significantly lower in the EB group compared to the OIL controls at both ages examined. Expression was not significantly altered in any of the other groups. (Scale bar = 500 μm, **P ≤ 0.005).