Kisspeptin immunolabeling in the anterior hypothalamus. Fluorescence photomicrographs showing Kiss immunoreactive (-ir) labeling in the AVPV of the hormone primed females (Experiment 1). (A) Kiss-ir was localized to extended lengths of fibers within and surrounding the AVPV. (B) Kiss-ir fibers (blue arrows) were readily visible and quantifiable at high magnification with the confocal microscope. The density of Kiss immunostaining was significantly lower in the EB and MID DPN (1 mg/kg) treated groups compared to the control animals. (3v = 3rd ventricle, ox = optic chiasm, (A) Scale bar = 50 μm, (B) Scale bar = 25 μm, Means ± S.E.M., *P ≤ 0.05, **P ≤ 0.005).