Effects of ovariectomy and estradiol treatment on excess SAT and IAAT. Excess SAT and excess IAAT were calculated with respect to the chow-fed intact group (CH-Intact, y-axis = 0), as described in the text. The left panel shows chow-fed rats that were either ovariectomized (CH-OVX; open squares) or ovariectomized and estradiol-treated (CH-OVX-E2; open triangles). The right panel shows Ensure-fed rats that were sham operated (EN-Intact, filled circles), ovariectomized (EN-OVX, filled squares), or ovariectomized and estradiol-treated (EN-OVX-E2, filled triangles). Data are means ± SEM and were analyzed by planned comparisons, with an experiment-wide significance of P < 0.05, as described in the text. *different from CH-Intact; ǂOVX different from OVX-E2, same diet group; ϕEN-OVX different from EN-Intact.