Changes in Foxl2 messenger RNA levels in the sex-changing gonads induced by aromatase inhibitor (AI) treatment. The copy number of Foxl2 was calibrated by tissue weight. Each bar represents mean ± standard error of mean as determined by a real time quantitative polymerase chain reaction assay. Gonadal stages of sex change are described in the Material and methods section of the article. Data points not sharing the same alphabets are shown to significantly different by Turkey-Kramer multiple comparison test. Numbers in parentheses indicated sample size.