Figure 4

Localization of Foxl2 protein by immunofluorescence in three-spot wrasse gonad sections. Red represents Foxl2 protein, whereas blue represents the nucleus. (A-D) Section of an ovary of a female fish. (D) High magnification of the area inside the rectangle shown in (C). The arrowhead indicates a positive signal in granulosa cell (GC) and an arrow indicates theca cells (Th) that have no signals. (E-H) Sections of the testis of an initial phase male. (H) High magnification of the area inside the rectangle shown in (G). (I-L) Sections of the testis of a terminal phase male. (L) High magnification of the area inside the rectangle shown in (K). O: oocyte; GC: granulosa cell; Th: Theca cell; SG: spermatogonia; SC: spermatocyte; ST: spermaid; SZ: spermatozoa. Bar = 50 μm.