Sequence and phylogenetic analysis of Foxl2 proteins. (A) Sequence alignment of Foxl2 proteins. Forkhead domain is underlined. The boxed peptide sequences were used for the synthesis of antigens of wrasse Foxl2. The identification of wrasse Foxl2 sequence with other sequences is indicated in percentage against each sequence. Amino acids identical to those in other species are indicated by asterisks (*). Colon (:) indicates conserved substitution and dot (.) indicates semi-conserved substitution. (B) A phylogenetic tree comprising the subfamily of Foxl2 proteins was constructed using neighbour-joining method. GenBank Accession Numbers for the aligned proteins are presented with species name. The tree was rooted using zebrafish Foxl1 as outgroup. The values are bootstrap probabilities estimated by 1000 replications. Horizontal line indicates genetic distance.