Fig. 7

Experimental design (A) and schematic diagram (B) of the analysis of key regulators of IGF1 signaling pathways in the liver of Pappa2^ko/ko and Pappa2^wt/wt mice (males and females) treated with rhGH, rhIGF1 and rhPAPP-A2 for 30 days, from PND5 to PND35. Phosphorylated forms of IRS1 (C), PI3K (D), AKT (E), mTOR (F), GSK3β (G), ERK2 (H) and AMPKα (I) are represented as mean ± S.E.M (n = 6 per experimental group). Representative immunoblots (J) are also shown. See Table S2 and Figures S4 and S6 for additional information. Two-way ANOVA and Tukey-corrected tests: */**/***p < 0.05/0.01/0.001 versus respective saline-treated males and females; ^#/##p < 0.05/0.01 versus respective wt/wt males and females