Fig. 6

Experimental design (A) and schematic diagram (B) of the analysis of circulating hormone concentrations, and liver IGF1 system gene expression in Pappa2^ko/ko and Pappa2^wt/wt mice (males and females) treated with rhGH, rhIGF1 and rhPAPP-A2 for 30 days, from PND5 to PND35. Concentrations of total IGF1 (C) and IGFBP5 (D) in plasma, and relative Igf1 (E), Ghr (F), Igf1r (G), Igfbp3 (H), Igfbp5 (I), Igfals (J), Stc1 (K) and Stc1 (L) mRNA levels are represented as mean ± S.E.M (n = 8–10 per experimental group). See Table S1 for additional information. Two-way ANOVA and Tukey-corrected tests: */**/***p < 0.05/0.01/0.001 versus respective saline-treated males and females; ^#/##/###p < 0.05/0.01/0.001 versus respective wt/wt males and females