Fig. 4

Key sensors of IGF1 signaling pathways in the hypothalamus, the pituitary gland and the liver of Pappa2^ko/ko and Pappa2^wt/wt mice (males and females) in adulthood. Hypothalamus: phosphorylated forms of PI3K (A), AKT (B), mTOR (C), GSK3β (D), ERK1 (E), ERK2 (F), AMPKα (G) and GS (H). Pituitary gland: phosphorylated forms of GSK3β (J), AMPKα (K), ERK1 (L) and ERK2 (M). Liver: phosphorylated forms of PI3K (O), AKT (P), mTOR (Q), GSK3β (R), ERK1 (S), ERK2 (T), AMPKα (U) and GS (V). Data are represented as mean ± S.E.M (n = 6 per experimental group). Representative immunoblots (I, N and W) are also shown. See Table S2 and Figures S1-S3 for additional information. Two-way ANOVA and Tukey-corrected tests: *^/**^/***p < 0.05/0.01/0.001 versus respective wt/wt males and females; ^#/##/###p < 0.05/0.01/0.001 versus respective wt/wt and ko/ko males