Fig. 3

IGF1 system gene expression in the hypothalamus, the pituitary gland and the liver of Pappa2^ko/ko and Pappa2^wt/wt mice (males and females) in adulthood. Hypothalamus: relative Pappa2 (A), Ghrh (B), Ghih-Sst (C), Igf1 (D), Igf1r (E), Igfbp3 (F), Igfbp5 (G), Igfals (H) and Stc1 (I) mRNA levels. Pituitary gland: relative Pappa2 (J), Gh (K), Ghrhr (L), Igf1 (M), Igf1r (N), Igfbp3 (O), Igfbp5 (P), Igfals (Q) and Stc1 (R) mRNA levels. Liver: relative Ghr (S), Igf1 (T), Igf1r (U), Igf2 (V), Igfbp3 (W), Igfbp5 (X), Igfals (Y) and Stc1 (Z) mRNA levels. Data are represented as mean ± S.E.M (n = 8–10 per experimental group). See Table S1 for additional information. Two-way ANOVA and Tukey-corrected tests: *^/***p < 0.05/0.001 versus respective wt/wt males and females; ^##/###p < 0.01/0.001 versus respective wt/wt and ko/ko males