Fig. 4

Neurogenic-like effects induced by different intensities of ECS treatment in adolescent and adult female rats. Quantitative analysis of a, b Ki-67 and c, d NeuroD + cells in the left dentate gyrus of adolescent or adult female rats. Data represents mean ± SEM of the number of + cells. Individual values are shown for each rat (symbols). The quantification was done in every 8th section through the entire extent of the hippocampal dentate gyrus and multiplied by the sampling factor 8 to provide an estimation of the total number of + cells per marker. One-way ANOVAs followed by Dunnett's multiple comparisons tests: ***p < 0.001 vs. SHAM (S). Representative images for each treatment group showing Ki-67 (brown labeling in the blue granular layer) and NeuroD (dark blue labeling in the blue granular layer) + cells acquired with a light microscope (40× objective lens) connected to a digital camera