Fig. 3

Adult NHP brain has sex bias in PON2 isoforms expression in STR and SN regions. STR (n = 5) and SN (n = 4). Protein expression of PON2 isoforms, i.e., 39 kDa and 41 kDa in STR (A, B) and SN (C, D), respectively. Representative blot showing PON2 isoforms expression in STR (E), and SN (F). Corresponding image of total protein in STR (G) and SN (H). Optical density of UCP5 and UCP4 bands were normalized to total protein per lane. The normality of infant male and female data was confirmed by a Shapiro–Wilk test. 39 kDa PON2 STR Male (W = 0.84, p = 0.17) Female (W = 0.97, p = 0.87); 41 kDa PON2 Male (W = 0.95, p = 0.77) Female (W = 0.92, p = 0.54); 39 kDa PON2 Male (W = 0.92, p = 0.58) Female (W = 0.91, p = 0.51); 41 kDa PON2 SN Male (W = 0.87, p = 0.33) Female (W = 0.95, p = 0.73). Data were expressed as mean ± SEM. Male and female data in each region was compared by two-tailed unpaired Student's t test. 39 kDa PON2 STR (two-tailed t (6) = 3.75, p = 0.0095), 41 kDa PON2 STR (two-tailed t (8) = 4.87, p = 0.0012), 39 kDa PON2 SN (two-tailed t (8) = 0.03, p = 0.97), 41 kDa PON2 SN (two-tailed t (6) = 2.72, p = 0.034). Asterisks indicate statistical significance in comparison with vehicle group, *p < 0.05, **p < 0.01, ***p < 0.001