Fig. 2

Young NHP brain has no sex bias in UCP4 and UCP5 expression in STR and SN regions (n = 5). Protein expression of UCP4 and UCP5 in STR (A, B) and SN (C, D), respectively. Representative blot showing UCP4 and UCP5 expression in STR (E, G), and SN (F, H) region. Corresponding image of total protein in STR (I) and SN (J). Optical density of UCP5 and UCP4 bands were normalized to total protein per lane. The normality of Infant male and female data was confirmed by a Shapiro–Wilk test. UCP4 STR Male (W = 0.86, p = 0.25) Female (W = 0.96, p = 0.81); UCP5 STR Male (W = 0.78, p = 0.057) Female (W = 0.84, p = 0.18); UCP4 SN Male (W = 0.93, p = 0.65) Female (W = 0.86, p = 0.25); UCP5 SN Male (W = 0.88, p = 0.35) Female (W = 0.93, p = 0.64). Data were expressed as mean ± SEM. Male and female data in each region was compared by two-tailed unpaired Student's t test. UCP4 STR (two-tailed t (8) = 0.99, p = 0.34), UCP5 STR (two-tailed t (8) = 1.85, p = 0.10), UCP4 SN (two-tailed t (8) = 0.73, p = 0.48), UCP5 SN (two-tailed t (8) = 2.23, p = 0.055)