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Table 4 Summary of statistical analysis of the patch clamp study

From: Sexual differences in locus coeruleus neurons and related behavior in C57BL/6J mice

Shapiro–Wilk normality test (W, P value, Passed normality test?)

 
 

Male

Female

 

Capacitance (pF)

0.9381

0.4329

Yes

0.9762

0.9262

Yes

 

Resistance (MΩ)

0.9726

0.9235

Yes

0.9438

0.3980

Yes

 

Resting potential (Vm)

0.8819

0.0757

Yes

0.9299

0.1539

Yes

 

Inward-rectifier potassium channels (IRK)

0.9469

0.6795

Yes

0.9563

0.7742

Yes

 

AP amplitude (mV)

0.9378

0.4288

Yes

0.9596

0.5650

Yes

 

Half-width (ms)

0.8983

0.1268

Yes

0.8688

0.0112

No

 

Threshold (mV)

0.8960

0.1177

Yes

0.9664

0.6768

Yes

 

AHP amplitude (mV)

0.8480

0.0269

No

0.9406

0.2461

Yes

 

Injected current (pA)

0.8698

0.0650

Yes

0.9132

0.2659

Yes

 

Firing frequency (Hz)

0.8979

0.1253

Yes

0.9389

0.4426

Yes

 

Voltage (mV)

0.9885

0.9851

Yes

0.9860

0.9770

Yes

 

Unpaired Student’s t-test (t(df))

  

Capacitance (pF)

t(27) = 2.19*

  

Resistance (MΩ)

t(27) = 1.90

  

Resting potential (Vm)

t(31) = 1.16

  

AP amplitude (mV)

t(30) = 0.98

  

Threshold (mV)

t(31) = 0.66

  

Injected current (pA)

t(21) = 3.95***

  

Mann–Whitney U test (U)

  

Half-width (ms)

U = 120.5

  

AHP amplitude (mV)

U = 108

  

Repeated-measures ANOVA (F(df. residual))

 

Sex

Current

Sex x Current

Inward-rectifier potassium channels (IRK)

F(1, 19) = 4.96*

F(7,133) = 178.09***

F(7,133) = 5.06***

Firing frequency (Hz)

F(1, 16) = 5.06*

F(12,192) = 78.80***

F(12,192) = 2.17*

Voltage (mV)

F(1, 19) = 1.09

F(5, 95) = 180.26***

F(5, 95) = 7.86***

  1. df, degrees of freedom
  2. *p < 0.05; ***p < 0.001
  3. Values in bold indicate statistically significant results