Sexual differences in locus coeruleus neurons and related behavior in C57BL/6J mice

Table 3 Summary of statistical analysis of LC morphology studies

Shapiro–Wilk normality test (W, P value, Passed normality test?)
	Male	Female
Number of TH cells (DAB)	0.8148 0.1065 Yes	0.9918 0.9856 Yes
Number of TH cells (IF)	0.9817 0.9433 Yes	0.9283 0.5851 Yes
AUC of TH cells distribution	0.9090 0.4614 Yes	0.7954 0.0743 Yes
Somatic volume (mm³)	0.9421 0.6806 Yes	0.8673 0.2556 Yes
Somatodendritic volume (mm³)	0.9288 0.5882 Yes	0.9480 0.7231 Yes
Dendritic volume (mm³)	0.9440 0.6947 Yes	0.9195 0.5268 Yes
AUC of Somatic distribution	0.9382 0.6529 Yes	0.8328 0.1460 Yes
AUC of Somatodendritic distribution	0.8018 0.0838 Yes	0.9155 0.5016 Yes
AUC of Dendritic distribution	0.7903 0.0673 Yes	0.8311 0.1417 Yes

Unpaired Student’s t-test (t_(df))
Number of TH cells (DAB)	t₍₈₎ = 2.51*
Number of TH cells (IF)	t₍₈₎ = 2.44*
AUC of TH cells distribution	t₍₈₎ = 2.89*
Somatic volume (mm³)	t₍₈₎ = 1.08
Somatodendritic volume (mm³)	t₍₈₎ = 3.06*
Dendritic volume (mm³)	t₍₈₎ = 3.47**
AUC of Somatic distribution	t₍₈₎ = 0.91
AUC of Somatodendritic distribution	t₍₈₎ = 1.61
AUC of Dendritic distribution	t₍₈₎ = 2.61*

TH, Tyrosine hydroxylase; DAB, 3,3′-diaminobenzidine tetrahydrochloride; IF, immunofluorescence; AUC, area under the curve; df, degrees of freedom
*p < 0.05; **p < 0.01
Values in bold indicate statistically significant results

ISSN: 2042-6410