Sexual differences in locus coeruleus neurons and related behavior in C57BL/6J mice

Table 2 Summary of statistical analysis of cognitive and sensorial behaviors

Shapiro–Wilk normality test (W, P value, Passed normality test?)
		Male				Female
Total distance (AU)		0.9415 0.5703 Yes				0.8977 0.2065 Yes
Total distance (AU)		1 min 0.9190 0.3485 Yes		10 min 0.9717 0.9064 Yes		1 min 0.9337 0.4849 Yes		10 min 0.9529 0.7032 Yes
Number of interactions		0.9701 0.8918 Yes				0.8965 0.2002 Yes
Identical object preference (%)		0.9419 0.5738 Yes				0.9505 0.6746 Yes
STM Discrimination index With two different objects		0.9456 0.6163 Yes				0.9461 0.6231 Yes
LTM Discrimination index With two different objects		0.9664 0.8559 Yes				0.9433 0.5903 Yes
STM Discrimination index With similar objects		0.9270 0.4193 Yes				0.9311 0.4589 Yes
Nociceptive response (%)	0.16 g	0.4 g	0.6 g		1 g	0.16 g	0.4 g		0.6 g	1 g
	0.2359 < 0.0001 No	0.3512 < 0.0001 No	0,5436 < 0,0001 No		0,4330 < 0,0001 No	0,3512 < 0,0001 No	0,3512 < 0,0001 No		0,5804 < 0,0001 No	0,5804 < 0,0001 No
	1.4 g	2 g	4 g		6 g	1.4 g	2 g		4 g	6 g
	0,4954 < 0,0001 No	0,4954 < 0,0001 No	0,5804 < 0,0001 No		0,6076 < 0,0001 No	0,6265 < 0,0001 No	0,5824 < 0,0001 No		0,6710 < 0,0001 No	0,6710 < 0,0001 No
	8 g	10 g				8 g	10 g
	0,4954 < 0,0001 No	0,6375 < 0,0001 No				0,7011 < 0,0001 No	0,5824 < 0,0001 No
Paw withdrawal (s)		0.8804 0.0265 No				0.8522 0.0147 No

Unpaired student’s t-test (t_(df))
Total distance (AU)	t₍₁₈₎ = 1.05
Male total distance (AU) vs 1 min	t₍₁₈₎ = 2.14*
Female total distance (AU) vs 1 min	t₍₁₈₎ = 2.21*
Identical object preference (%)	t₍₁₈₎ = 0.29
Number of interactions	t₍₁₈₎ = 2.50*
STM discrimination index With two different objects	t₍₁₈₎ = 0.34
LTM discrimination index With two different objects	t₍₁₈₎ = 0.80
STM discrimination index With similar objects	t₍₁₈₎ = 3.11**

Mann–Whitney U test (U)
Paw withdrawal (s)	U = 75.50*

Repeated-measures ANOVA (F_{(df. residual)})
	*Sex*	*Force (g)*	*Sex x Force (g)*
Nociceptive response (%)	F_{(1, 38)} = 1.09	F_{(9, 342)} = 2.70**	F_{(9, 342)} = 0.72

AU, Arbitrary Units; STM, Short-term memory; LTM, Long-term memory; df, degrees of freedom. *p < 0.05, **p < 0.01
Values in bold indicate statistically significant results

ISSN: 2042-6410