Fig. 4

Evaluation of the passive properties and excitability of LC neurons in male and female mice. Population graphs depicting differences in a the membrane capacitance, b a slight difference in the membrane resistance and c similar membrane resting potentials. d Representative example and graph of the IRK currents. e Representative example of the action potential (AP) and corresponding parameters, such as the amplitude, half-width, threshold and after hyperpolarization potential (AHP). f Representative examples of the voltage responses of identified LC neurons to current injection of + 100 and − 100 pA, respectively. g Rheobase. h Graph showing the activity driven in response to the injection of positive currents (+ 25 pA steps). i Graph showing the voltage deflections in response to the injection of negative currents (− 25 pA steps). The data are presented as the mean ± SEM of n = 5 mice per group: *p < 0.05, **p < 0.01 ***p < 0.001 vs male. ANOVA (sex factor): ^#p < 0.05, ^###p < 0.001