Fig. 3

Quantification of TH⁺ cells and the LC volume in male and female mice. a Representative images of the LC in male and female mice stained for TH by DAB, and b quantification of the number of TH⁺ cells in both groups. c Representative confocal images of the area occupied by the soma (white line) and/or dendrites (yellow line) outlined for volume estimation. d Quantification of the number of TH⁺ cells detected by immunofluorescence in the entire LC. e Graph depicting the distribution of TH⁺ cells along the rostrocaudal axis of the LC and f its representation as the AUC. g The volume occupied by the soma, h somatodendrites and i dendrites of the entire LC. j Graph depicting the distribution of TH⁺ dendrites along the rostrocaudal axis of the LC and k its representation as the AUC. The data are presented as the mean ± SEM of n = 5 animals per group for DAB and another 5 animals per group for inmunofluorescence: *p < 0.05, **p < 0.01 vs male. Scale bars = 100 μm. IV, fourth ventricle; TH, Tyrosine Hydroxylase; DAB, 3,3′-diaminobenzidine tetrahydrochloride; AUC, area under curve