Fig. 1

Evaluation of anxiety, well-being and depressive-like behavior in male and female mice. The results of anxiety-like behavior expressed as a the relative time spent in the open arms of the EPM by male and female mice. b Percentage of time spent in the lit compartment in the light–dark test. c Percentage of time spent in the central zone in the OFT, along with representative heatmaps of activity. d Graph depicting the material burrowed (in grams) and e the AUC of the material burrowed over time. f Time spent grooming in the splash test. Evaluation of the depressive-like behavior expressed as g immobility time in the TST and h FST. The data are presented as the mean ± SEM of n = 7–10 mice per group: *p < 0.05, **p < 0.01 vs male. From b to h females were in proestrus and estrus (P/E) stages. AUC, area under the curve