Fig. 7

Sex differences in sphingolipid content profile and CER metabolism in renal cortex tissue from males and females in response to a HFD. Sum of the quantitative amount of FA species within a class after normalization (DNA content from tissue samples). A, B Changes in SM profile in males and females fed a HFD or a LFD. C, D Changes in CER profile in males and females fed a HFD or a LFD. Statistical analyses were performed using multiple t-tests with P values corrected for multiple comparison using the Bonferroni method. Data are presented as means ± SEM. n = 4–6 in each group. E–F Real-time quantitative PCR analysis for CerS2, CerS5, CerS6, Acer2 and Acer3 genes. mRNA expression was performed with kidney tissue from LFD and HFD female and male mice and normalized against 18S. Statistical analyses were performed using two-way ANOVA followed by Tukey post-test. *p < 0.05; **p < 0.01; ***p < 0.001. Data are presented as means ± SEM