Fig. 6

Sex differences in renal lysosomal dysfunction in males and females in response to a HFD. A Representative photomicrograph (original magnification × 40) showing LAMP1–positive staining on intracellular vacuoles in males and females fed a HFD or a LFD. B Related quantitative analysis of LAMP1-positive staining. C–E Real-time quantitative PCR analysis for LAMP1, Cathepsin D and p62 genes. mRNA expression was performed with kidney tissue from LFD and HFD female and male mice and normalized against 18S. Statistical analyses were performed using two-way ANOVA followed by Tukey post-test. *p < 0.05; **p < 0.01. Data are presented as means ± SEM