Fig. 1

Sex differences in metabolic parameters in response to HFD. Male and female C57BL/6J mice were fed with either HFD or LFD for 16 weeks. A Relative increase in BW. Statistical analyses were performed using two-way ANOVA followed by Tukey post-test. *p < 0.05 HFD vs LFD male and ^#p < 0.05 HFD vs LFD female. n = 6 in each group. B Plasma leptin level. C Fasting blood glucose and D plasma insulin levels were assessed in male and female mice fed a LFD or a HFD at week 16. E HOMA-IR index calculation. F Plasma FFA and G cholesterol levels. H Representative photomicrographs (original magnification ×400) of H&E staining illustrating hepatic steatosis from liver sections in males and females fed a LFD or a HFD. I Semiquantitative grading of hepatic steatosis in each group. Statistical analyses were performed using two-way ANOVA followed by Tukey post-test. *p < 0.05; **p < 0.01; ***p < 0.001. Data are presented as means ± SEM