Sex differences in distribution and identity of aromatase gene expressing cells in the young adult rat brain

Table 2 Co-expression of cell markers with Cyp19a1 in different brain regions

Region		Acb		BNST		MPR		VMH		MeP		PCo		AHi
Sex		M	F	M	F	M	F	M	F	M	F	M	F	M	F
Gad1 co-localization
Median		28.6	10.3	53.1	27	47	46.3	11.8	5.9	42.2	26.4	17.4	18.8	20	21.4
25th percentile		13	6.1	48.7	7.9	35.8	32.7	11.1	3.6	32.7	5.7	16.4	8.9	15.1	13.8
75th percentile		33.6	16.7	55.6	48.2	48.6	54.7	16.8	11	54.1	47.1	17.5	27.7	30	25.9
N		5	4	5	4	5	4	5	4	5	4	5	4	5	4
Mann–Whitney U test	p	0.19		0.286		0.905		0.19		0.413		0.905		0.73
Mann–Whitney U test	r_b	0.6		0.5		− 0.1		0.6		0.4		0.1		0.2
Gad2 co-localization
Median		53	74.1	84.2	38.3	73.4	44.7	59.9	8.6	76.6	26.6	51.8	28.6	54.8	25
25th percentile		49	59.2	82.8	30.8	65	22.8	34.6	0.2	56.4	19.8	39.1	23.9	50.1	18.6
75th percentile		64.8	76	87.2	59	74.7	67.7	69	31.7	78.8	39	68.7	40.6	73.9	31.1
N		5	5	6	6	6	6	5	6	5	6	6	6	6	5
Mann–Whitney U test	p	0.421		0.065		0.132		0.082		0.017		0.132		0.052
Mann–Whitney U test	r_b	− 0.4		0.7		0.6		0.7		0.9		0.6		0.7
Slc17a7 co-localization
Median		10.3	1							0.3	2.3	40.1	73	35.3	74.7
25th percentile		8.1	0.3							0.06	0	33	53.5	9.5	52.6
75th percentile		14.6	5.4							0.4	5.4	55.2	79	48.9	82.5
N		4	4							5	4	5	4	5	4
Mann–Whitney U test	p	0.2								0.901		0.19		0.286
Mann–Whitney U test	r_b	0.6								− 0.1		− 0.6		− 0.5
Slc17a6 co-localization
Median				0.3	0	3	1.8	4.9	7.2	0.8	0	1	0.4	0.7	0.1
25th percentile				0	0	2.7	0	1.5	0.5	0	0	0.5	0	0	0
75th percentile				0.4	0	4.1	7.3	7.8	11.1	0.9	0	2.4	0.5	1.4	1.1
N				5	5	5	5	5	5	5	5	5	5	5	5
Mann–Whitney U test	p			0.48		1		1		0.48		0.398		1
Mann–Whitney U test	r_b			0.3		0		0		0.3		0.4		0
Slc1a3 co-localization
Median		1.6	0.5	5.7	0	5.8	2.4	7.6	0	4.5	1.1	3.9	0.3	5.2	0.8
25th percentile		0.9	0.4	4.8	0	5	0.6	4.7	0	2	0.6	3.1	0	4.5	0
75th percentile		2.4	1.4	6.1	0	6.9	3.1	11.7	0	8.7	1.6	5.3	0.9	6.9	4.5
N		4	3	4	5	4	5	4	5	4	5	4	5	4	5
Mann–Whitney U test	p	0.229		0.015		0.111		0.081		0.063		0.037		0.268
Mann–Whitney U test	r_b	0.7		1		0.7		0.7		0.8		0.9		0.5

The sex differences in the main regions of interest are presented as the percentage of Cyp19a1⁺/cell marker⁺ nuclei from all Cyp19a1⁺ nuclei in a region. As no Slc17a7 was expressed in the BNST, MPR or VMH nor Slc17a6 in the Acb, no co-expression analyses were performed in those regions. p: uncorrected p-value of the Mann–Whitney U test, bold if uncorrected p < 0.05. r_rb: effect size based on rank-biserial correlation. Acb nucleus accumbens; AHi amygdalo-hippocampal nucleus, BNST bed nucleus of the stria terminalis, f female, m male; MeP posterior part of the medial amygdaloid nucleus, MPR medial preoptic region, PCo posterior part of the cortical amygdaloid nucleus, VMH ventromedial hypothalamic nucleus

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