Fig. 1

Design of the study. Brain sections of male and female rats killed at post-natal week 10 were analyzed by using gene-specific probes and performing fluorescent in situ hybridization. The slides were scanned with a digital full slide scanner at 20 × mean magnification. Quantification of Cyp19a1⁺ as well as cell marker⁺ cells was performed using CellProfiler. The right panel shows Cyp19a1 (red) predominantly surrounding the DAPI-stained nucleus (blue) of the expressing cells. Some Cyp19a1⁺ nuclei are indicated with a white arrow. Abbreviations: PNW: post-natal week. The figure was created with BioRender.com