Fig. 4

Analysis of infiltrating CD8⁺ T-cell transition states in male and female samples. A Pseudotime-ordered analysis and density-distribution map of infiltrating CD8⁺ T-cells in tumors and adjacent normal kidneys of males and females. B Two-dimensional plots showing the change of expression scores for genes related to T-cell exhaustion and dysfunction along with the pseudotime. C Two-dimensional plots showing the dynamic expression of exhaustion and cytotoxicity genes during the CD8⁺ T-cell transitions along the pseudotime in male (blue) and female (red) samples. D The exhausted status and cytotoxic function of tumor-infiltrating CD8⁺ T-cells assessed by flow cytometry in male (n = 10) and female tumor (n = 10) samples