Fig. 5

PKMζ knockout alters LTD in mice in the nucleus accumbens core in a sex-specific manner. LTD induction in female mice by a shorter protocol of LFS (1-Hz train of paired stimuli for 5 min) is facilitated in PKMζ knockout animals compared to wildtype controls (A; n = 12–13/group). Change in fEPSP slope over 1-h post-LFS shows PKMζ knockout facilitates LTD in female mice compared to wildtype controls (B; n = 12–13/group). LTD induction in male mice by a shorter protocol of LFS (1-Hz train of paired stimuli for 5 min) is blunted in PKMζ knockout animals compared to wildtype controls (D; n = 10–12/group). Change in fEPSP slope over 1-h post-LFS shows PKMζ knockout blunts LTD in male mice compared to wildtype controls (E; n = 10–12/group). Following the longer LFS induction protocol (1-Hz train of paired stimuli for 10 min), there is no effect of PKMζ knockout on LTD induction in either male or female mice (G; n = 10–14/group; J; n = 9–12/group). Change in fEPSP slope over 1-h post-LFS reveals robust LTD in both wildtype and PKMζ knockout mice (females: H; n = 10–14/group; males: K; n = 9–12/group). Representative pre- and post-LFS traces (C, F, I, L). *p < 0.05; **p < 0.01 effect of genotype, ^#p < 0.01 significant LTD