Fig. 2

Sex and age interact to influence glucose intolerance in 3xTg-AD mice. A–D Blood glucose levels were measured following overnight fasting (t = 0), then mice were subjected to a glucose tolerance test (GTT). Mice were injected with glucose challenge and blood glucose levels were measured at 15, 30, 60, 90, and 120 min post-injection. Graphs show results of the glucose tolerance test in 3xTg-AD male and female mice at A 3, B 6, C 9, and D 12 months of age. E Area under the curve for GTT testing was computed as a measure of total glucose exposure. n = 4–10/group. *p < .05 vs. opposite sex of same age, **p < 0.01 vs. opposite sex of same age, ****p < 0.0001 vs. opposite sex of same age, ^ = p < 0.05 vs. same-sex 3 month; ^^^^ = p < 0.0001 vs. same-sex 3 month. GTT = glucose tolerance test, AUC = area under the curve, min = minutes. Data are presented as mean ± SEM