Fig. 8

miR-30a was expressed in the bronchiolar epithelial cell population in the lung upon exposure to hyperoxia: In situ, hybridization in representative WT lung sections for miR-30a identified the spatial localization of miR-30a in the lung in hematoxylin-stained sections, with consecutive sections stained with eosin and hematoxylin for lung structure. A Tiled H&E image of the entire lung section subjected to miR-30a in situ hybridization. Black bars = 2 mm. B Representative lung regions (40×) focused on bronchioles to discern miR-30a expression pattern in the lung. Black bars = 25 µm. C Magnified (60×) images of the bronchioles showing the punctate light blue staining of miR-30a expression in the bronchial epithelial cells, highest in the WT female lung exposed to hyperoxia. Black bars = 5 µm. D Representative lung section from a miR-30a^−/− mouse showing absence of punctate staining. Black bar = 25 µm. E Quantitation of miR-30a staining in the lung in the different anatomical regions (bronchioles, lung parenchyma and vessel) for males (M) or females (F) exposed to room air (grey circles) or hyperoxia (black squares). N = 3/group