Fig. 6

Validation of RNA-Seq data by qRT-PCR.A Examples of differentially expressed genes that were upregulated in miR-30a^−/− female but downregulated in the WT female by RNA-Seq. We selected target genes that were known miR-30a targets and down-regulated in the female WT lung but upregulated in the miR-30a^−/− lung for validation by qRT-PCR. B Chl1 mRNA expression C Cd109 mRNA expression. Values are mean ± SD. Independent biological replicates in each group are shown (n = 3–4/group). Statistical analysis was performed using 3-way ANOVA. Significant differences between groups are shown by **P < 0.01, and ***P < 0.001. D Cell-type specific expression of Chl1 and Cd109 from the LungMAP database. UMAP plots showing different lung cell sub-populations and expression of Chl1 and Cd109 in basal cells. E Expression levels of Chl1 and Cd109 (Fold change and -log ₁₀ p value) in the lung cell types. The cell groups with the highest expression are depicted here. PNEC: pulmonary neuroendocrine cell; MEC: myoepithelial cell; Cap1: general capillary endothelial cell; pMAC: tissue resident macrophage; mDC1: classical dendritic cell subset 1