Fig. 4

Loss of miR-30a lead to impairment of distal lung vascular development in male and female mice: pulmonary vascular density was assessed in the distal lung using immunohistochemistry for von Willebrand factor (vWF). Brown-stained vessels (< = 50um in diameter) were counted at 20× magnification per high power field (hpf), in 10 randomly chosen fields per lung in neonatal WT and miR-30a^−/− mice exposed to room air or hyperoxia (95% FiO₂, PND1-5) on PND 21. A Representative immunostained images for von Willebrand factor in male and female WT and miR-30a^−/− mice in the normal air and hyperoxia groups at 20 × magnification. Black bars = 50 µm. B Values for counts of pulmonary vessels, are represented as mean ± SE. Independent biological replicates in each group are shown (n = 3–6/group). Significant differences between groups are shown by *P < 0.05, **P < 0.01, and ***P < 0.001. Statistical analysis was performed using 3-way ANOVA