Fig. 3

Sex-specific differences in macrophage counts were lost in miR-30a^−/− mice in the setting of neonatal hyperoxia exposure. A Representative lung sections from male and female WT and miR-30a^−/− mice in the room air and hyperoxia groups at 40 × magnification stained for macrophages using an F4/80 antibody. Black bars = 50 µm. B Macrophage quantitation by immunohistochemistry for F4/80 in neonatal WT and miR-30a^−/− exposed to room air or hyperoxia (95% FiO₂, PND1-5) on PND21. Values are mean ± SE. Independent biological replicates in each group are shown (n = 3–7/group). Significant differences between groups are shown by *P < 0.05, **P < 0.01, and ***P < 0.001. Statistical analysis was performed using 3-way ANOVA