Fig. 5From: Zebrafish gonad mutant models reveal neuroendocrine mechanisms of brain sexual dimorphism and male mating behaviors of different brain regionsRT‒qPCR was performed to validate the RNA transcriptome data in different brain region samples. A–D nr2e3, dio2, cyp19a1b, and igf1 in the forebrain; E–H egr2b, gh1, slc25a18, klf3, and dio2 in the midbrain; I–L sox19a, ddx43, npas2, klf3, and shisa19 in the hindbrain. Error bars represent the mean ± SD; bars with different letters are significantly different from each other, P < 0.05. Brain samples were n = 5 in biological triplicatesBack to article page