Fig. 4

Hormone concentrations of testosterone (T), 11-ketotestosterone (11-KT) and 17β-estradiol (E2) in the gonads, serum, and brain of control males, tdrd12^−/− fish, cyp17a1.^−/− fish, double knockout (KO) fish and control females. A–C T levels in the gonads, blood, and brain samples. D–F 11-KT levels in the gonads, blood, and brain samples. C E2 levels in the gonads, blood, and brain samples. For this assay, the numbers of gonadal, blood, and brain samples were n = 4, n = 6 and n = 8; Error bars represent the mean ± SEM. Bars with different letters are significantly different from each other, P < 0.05