Fig. 1

Courtship parameters of wild-type male, tdrd12^−/−, cyp17a1.^−/− and double knockout (KO) fish with wild-type females. Twelve individuals of each genotype were selected and randomly paired with wild-type females, and all pairs were exposed to light for 20 min before the mating partition was removed. A Frequency of intimate contact with wild-type females during the courtship period. B Duration of intimate contact time with females during the courtship period. C Average frequency of intimate contact with females per minute during the courtship period. D Average duration of intimate contact time with females per minute during the courtship period. E Representative mating behaviors of males of four genotypes with wild-type females. *Significant difference (P < 0.05); **significant difference (P < 0.01); ***significant difference P < 0.001