Fig. 2
Kidney tubulointerstitial fibrosis and tubular casts in male and female SHRSP rats. a Tubulointerstitial fibrosis (% collagen) within the renal cortex. b Mean number of tubular casts present per field of view (1.5 mm2). c Representative images of Masson’s trichrome staining showing collagen deposition (stained blue) and tubular casts. 0.5 × magnification: scale = 1 mm. 10 × and 20 × magnification: scale = 50 µm. Values represent mean ± SEM; N = 8–11/group. a Data analysed by two-way ANOVA with Sidak’s multiple comparison test; **P < 0.01. b *p < 0.05 and ***p < 0.001 via a Kruskal–Wallis test with Dunn’s multiple comparisons