Sex-specific IL-6-associated signaling activation in ozone-induced lung inflammation

Table 4 Two-way ANOVA for univariate and pairwise comparisons between gender, estrous cycle stages, and exposure for STAT3 expression

Univariate tests: dependent variable: STAT3
			df	F	Sig.	Partial η2
Analysis of gender and exposure	Exposure	FA	1, 52	0.031	0.862	0.001
	Exposure	Ozone	1, 52	4.220	0.045	0.075
	Sex	F	1, 52	0.152	0.698	0.003
	Sex	M	1, 52	2.217	0.143	0.041
Analysis of female estrous cycle stages and exposure	Estrous cycle stage	Non-proestrus	1, 24	1.916	0.179	0.074
	Estrous cycle stage	Proestrus	1, 24	16.113	0.001	0.402
	Exposure	FA	1, 24	0.087	0.770	0.004
	Exposure	Ozone	1, 24	32.414	< .0005	0.575
Pairwise comparisons: dependent variable: STAT3
			(I) Sex	(J) Sex	Mean difference (I–J)	95 % CI for difference
			(I) Sex	(J) Sex	Mean difference (I–J)	Lower bound	Upper bound
Analysis of gender and exposure	Exposure	FA	F	M	0.012	0.120	0.143
	Exposure	Ozone	F	M	0.135	0.003	0.267
	Sex	F	Ozone	FA	0.026	0.106	0.157
	Sex	M	FA	Ozone	0.098	0.034	0.230
Analysis of female estrous cycle stages and exposure	Estrous cycle stage	Non-proestrus	FA	Ozone	0.143	0.070	0.356
	Estrous cycle stage	Proestrus	Ozone	FA	0.414	0.201	0.628
	Exposure	FA	Proestrus	Non-proestrus	0.030	0.183	0.244
	Exposure	Ozone	Proestrus	Non-proestrus	0.588	0.375	0.801

ISSN: 2042-6410