Fig. 2

Immunofluorescence photomicrographs of sections through the proximal pars distalis (PPD) of the pituitary of the male (a) and female (b) Clarias batrachus showing tyrosine hydroxylase (TH) immunoreactive (ir) fibers (arrows). Semiquantitative image analysis of the (c) intensity of TH-ir and (d) percent fluorescent area occupied by TH-ir fibers in the PPD of the male and female. Open squares in the inset indicate the areas from where the measurements were collated. Note the significantly more TH-ir fibers in the PPD of female fish. *P < 0.05 and ***P < 0.0001 compared to males. Scale bar = 25 μm