Table 1 Primer sequences
From: Estrogens increase expression of bone morphogenetic protein 8b in brown adipose tissue of mice
Gene | Accession no. | Forward primer | Reverse primer | Reference |
|---|---|---|---|---|
Rn18s | NR_003278 | GTAACCCGTTGAACCCCATT | CCATCCAATCGGTAGTAGCG | Â |
B2m | NM_009735 | ATCCAAATGCTGAAGAACGG | CAGTCTCAGTGGGGGTGAAT | Â |
Hprt | NM_013556 | GCAGTACAGCCCCAAAAT | AACAAAGTCTGGCCTGTATCCAA | Â |
Ucp1 | NM_009463 | GGCCTCTACGACTCAGTCCA | TAAGCCGGCTGAGATCTTGT | [47] |
Bmp2 | NM_007553 | TGCTTCTTAGACGGACTGCG | CTGGGGAAGCAGCAACACTA | Â |
Bmp4 | NM_007554 | GAGCCATTCCGTAGTGCCAT | AACGACCATCAGCATTCGGT | Â |
Bmp5 | NM_007555 | ATGCCACCAACCATGCCATA | GCCACACGAACGTACTACCA | Â |
Bmp6 | NM_007556 | AACGCACACATGAATGCCAC | CCACAAGCTCTCACGACCAT | Â |
Bmp7 | NM_007557 | CGGGAGTTCCGGTTTGATCT | GCAAGAAGAGGTCCGACTCC | Â |
Bmp8b | NM_007559 | CAACCACGCCACTATGCAG | CACTCAGCTCAGTAGGCACA | Â |
Fgf1 | NM_010197 | GAAGCATGCGGAGAAGAACTG | CGAGGACCGCGCTTACA | [48] |
Fgf2 | NM_008006 | CAACCGGTACCTTGCTATGA | TCCGTGACCGGTAAGTATTG | [20] |
Fgf6 | NM_010204 | ACACACGAGGAGAACCCCTA | TGGAAGCTGGGCGTTGTGTA | Â |
Fgf9 | NM_013518 | TATCCAGGGAACCAGGAAAGAC | CAGGCCCACTGCTATACTGATAAA | [48] |
Fgf10 | NM_008002 | GCGGGACCAAGAATGAAGA | AGTTGCTGTTGATGGCTTTGA | [20] |
Fgf11 | NM_010198 | TTGTACAGCTCGCCACATTTC | GTAATTCTCAAAGACGCACTCCTT | [20] |
Fgf13 | NM_010200 | AATGAACAGCGAGGGATACTTG | ACTGATTCTTTGAATTTGCACTCA | [20] |
Fgf14 | NM_207667 | ACCCATCAGAACTTTTTACCCCT | ACATGGCAACTTCCAATGGCT | Â |
Fgf16 | NM_030614 | GGCCTGTACCTAGGAATGAATGA | TTCCCGGAAAACACATTCAC | [20] |
Fgf18 | NM_008005 | CATTCAAGTCCTGGGCCGTA | ATGAACACGCACTCCTTGCT | Â |
Fgf21 | NM_020013 | CTGGGGGTCTACCAAGCATA | CACCCAGGATTTGAATGACC | [49] |
Pparγ | NM_001127330 | CAAGAATACCAAAGTGCGATCAA | GAGCTGGGTCTTTTCAGAATAATAAG |  |