Figure 8
From: Estrogens increase expression of bone morphogenetic protein 8b in brown adipose tissue of mice
Effect of DES treatment on brown adipocyte gene expression in vitro . Primary cultured murine brown adipocytes were treated with or without 10 uM diethylstilbestrol (DES) for 24 h. (A) Representative picture of the primary murine brown adipocyte culture after 12 days of differentiation. (B) Ucp1, Pparγ, Bmp8b, and Fgf1 mRNA expression in primary brown adipocytes. Expression was normalized to hypoxanthine-guanine phosphoribosyltransferase (Hprt) with data from control adipocytes defined as ‘1’. Values are averages ± SEM; n = 4; *p < 0.05 (Mann-Whiney U test).