Figure 5

While BMPR2 gene expression is reduced, canonical BMP pathway activity is increased by exposure to E2. (A) A BMP responsive plasmid (pBRE system) studies: pBRE luciferase activity, a measure of BMP activity, is increased with exposure to increasing doses of E2. Uniform exposure to 50 ng of BMP4 in concert with exposure to MPP or varying doses of E2 occurred. Exposure to MPP, an ERα antagonist, resulted in reduced luciferase expression, while E2 exposure increased luciferase expression. (B) Consistent with this finding, gene expression of the canonical BMP signaling target (ID1) is increased with exposure to 10 nm E2 for 16 h.