Figure 2
From: BMPR2 expression is suppressed by signaling through the estrogen receptor
The BMPR2 promoter contains a functional estrogen receptor binding site. (A) Human BMPR2 promoter sequence (top line) containing an estrogen receptor binding site (boxed), used for gel-shift assay below, is conserved across species. (B) Gel-shift assay indicating that the estrogen receptor binding site is functional. BMPR2 probe sequence binds a protein (lane 1) of identical size to that bound by the canonical estrogen receptor binding site in Vitellogenin (lane 5). Binding to both the BMPR2 probe sequence and the Vitellogenin probe sequence can be competed away using cold BMPR2 probe sequence (lanes 3 and 4). Protein binding is not found when protein is omitted (lane 6) or with use of scrambled probe sequence (lane 2).