Fig. 3
Cellular senescence markers in kidneys of male and female SHRSP rats. a Senescence-associated β-galactosidase (SA-β-gal) activity within the renal cortex (% positive staining). Cells per field of view (FOV; 1.5 mm2) positive (+) for (b) p21, (C) p16INK4a and (d) γH2AX immunostaining. e Representative images of SA-β-gal activity in kidneys from all groups. p16INK4a, p21 and γH2AX immunostaining in the kidney interstitium and tubular cells of 8.5 Mo male SHRSP rats. Scale = 50 µm for all images. Values represent mean ± SEM; N = 8–11/group. a Data analysed via two-way ANOVA with a Sidak’s post hoc test. b–d *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 via a Kruskal–Wallis test with Dunn’s multiple comparisons